![]() ![]() A wrong streaking method can ruin your plate. If you are not skilled enough, then you would end up tearing through the agar, especially if you apply too much pressure on the agar. Training and technique are required as various growth media have various densities to the agar.The procedure can be a tedious process, especially if you don’t know the sample size before the lab work. You will be required to prepare the agar ahead of time.It requires huge storage space and there is a possibility that your incubator cannot accommodate a large volume of pet ri plate.It will not enable you to get a concentration count. The streak plate method does not work with high volumes of organisms.The streak plate method is convenient and hassle-free as you will be able to get visible individual colonies in a single petri plate for as long as you have a good streaking skill.Through the streak plate method, you will end up with a genetically identical individual colony, which makes it easy for you to grab and transfer colonies for a microscopic examination.It is the ideal method if you are doing general work with a certain type of microorganism.The streak plate method enables you to select and work with individual colonies.Repeat step four and go back to the area you streaked in the third quadrant of the petri plate and this time extend the streak into the fourth quadrant of the petri plate.Repeat step four and this time extend the streak into the petri plate’s third quadrant.Extend the streaks into the second quarter of the petri plate. Put the loop in the flame and let it cool.Gently streak the inoculating loop using a back and forth motion.Using the inoculating loop, pick an isolated colony from the agar plate and spread on the first quadrant of the petri plate.Wait for the loop to turn red indicating that it is already hot. The inoculating loop should be sterilized in the Bunsen burner by simply putting the loop into the flame.(4, 7) How is the streak plate method done? Isolation of the organism is a must in a mixed culture, especially if you need to thoroughly study the colony morphology of a particular organism. The purpose of the streak plate method is to produce an isolated colony of an organism on the agar plate. ![]()
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